Synthetic Biology:A Lab Manual Book PDF, ePub eBook

Join our eBook service for unlimited books & audiobooks. Get a free 7 day subscription! ↓↓↓↓↓


Book Descriptions:

Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students.

An inspiring foreword is written by another pioneer in the field, Harvard's George Church:
“Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed ‘A Lab Manual’ such as the one that you now hold in your hands.”

Contents:

• Introduction:
  • What is Synthetic Biology, Exactly?
  • The iGEM Outbreak
  • A Synthetic Biology Lab Manual
• Genes, Chromoproteins and Antisense RNAs:
  • E. coli DNA: Chromosomes, Plasmids and Copy Number
  • Coupling of Transcription and Translation in Bacteria
  • Promoter and Terminator for Transcription
  • Ribosome Binding Site (RBS)
  • Codon Bias
  • Chromoproteins
  • Small Regulatory RNAs (sRNAs)
• Lab Rooms and Equipment:
  • The Physical Lab Spaces
  • Equipment
• Safety is Priority #1:
  • Fires
  • Chemicals
  • Biological Safety and Disposal
  • Dangerous Equipment
• Lab Course Projects:
  • Time and Resources
  • Project Overview and Learning Objectives
  • The Lab Notebook
  • Lab Section 1. Preparation of Chemical Solutions and Agar Plates
  • Lab Section 2. Coloring Bacteria by Adding a Promoter to a Chromoprotein Gene
  • Lab Section 3: Rational Engineering of Chromoprotein Expression Level
  • Lab Section 4. Other Experiments
  • The “Dreaded” Exam
• Protocols:
  • Introduction
  • Protocol 1. Preparation of Solutions and Agar Plates
  • Protocol 2. Overnight Cultures with Antibiotics, and Glycerol Stocks
  • Protocol 3. BioBrick™ 3A Assembly and Gel Analysis
  • Protocol 4. Agarose Gel Electrophoresis
  • Protocol 5. Preparation of Competent E. coli Cells Using CaCl₂
  • Protocol 6. Transformation of CaCl₂-Competent E. coli Cells
  • Protocol 7. Bacterial Re-Streaking Techniques
  • Protocol 8. Lysis of E. coli Cells with Lysozyme
  • Protocol 9. Polymerase Chain Reaction (PCR)
  • Protocol 10. Inverse PCR Mutagenesis
  • Protocol 11. Colony PCR
  • Protocol 12. Gibson Assembly
• Advanced Methods:
  • Flow Cytometry and Cell Sorting
  • Recombination in Plasmids and the Chromosome
  • Electrocompetent Cells
• The International Genetically Engineered Machine (iGEM) Competition:
  • How to Start an iGEM Team
  • Uppsala iGEM 2011 — Show Color with Color
  • Uppsala iGEM 2012 — Resistance is Futile
  • Uppsala iGEM 2013 — Lactonutritious — It's Delicious
• Appendices

Readership: Students and researchers in biotechnology, cell/molecular biology and genetics.

Synthetic Biology:A Lab Manual by Josefine Liljeruhm

From reader reviews:

Chris Bynum:

The feeling that you get from Synthetic Biology:A Lab Manual is a more deep you searching the information that hide within the words the more you get thinking about reading it. It doesn't mean that this book is hard to be aware of but Synthetic Biology:A Lab Manual giving you excitement feeling of reading. The article writer conveys their point in selected way that can be understood simply by anyone who read this because the author of this e-book is well-known enough. This particular book also makes your current vocabulary increase well. Making it easy to understand then can go with you, both in printed or e-book style are available. We propose you for having this kind of Synthetic Biology:A Lab Manual instantly.

Geraldine Dube:

Spent a free time for you to be fun activity to complete! A lot of people spent their spare time with their family, or their particular friends. Usually they accomplishing activity like watching television, going to beach, or picnic inside the park. They actually doing ditto every week. Do you feel it? Do you wish to something different to fill your current free time/ holiday? Could be reading a book can be option to fill your free of charge time/ holiday. The first thing that you will ask may be what kinds of e-book that you should read. If you want to consider look for book, may be the e-book untitled Synthetic Biology:A Lab Manual can be excellent book to read. May be it may be best activity to you.

Bill Underhill:

Synthetic Biology:A Lab Manual can be one of your beginner books that are good idea. All of us recommend that straight away because this reserve has good vocabulary that will increase your knowledge in vocabulary, easy to understand, bit entertaining but nevertheless delivering the information. The copy writer giving his/her effort to set every word into pleasure arrangement in writing Synthetic Biology:A Lab Manual nevertheless doesn't forget the main level, giving the reader the hottest in addition to based confirm resource info that maybe you can be among it. This great information can certainly drawn you into new stage of crucial considering.

Jerry Ingle:

Your reading 6th sense will not betray a person, why because this Synthetic Biology:A Lab Manual book written by well-known writer whose to say well how to make book that can be understand by anyone who read the book. Written with good manner for you, still dripping wet every ideas and composing skill only for eliminate your own personal hunger then you still hesitation Synthetic Biology:A Lab Manual as good book not only by the cover but also by the content. This is one guide that can break don't determine book by its protect, so do you still needing a different sixth sense to pick this!? Oh come on your reading sixth sense already said so why you have to listening to another sixth sense.